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Well, it is hard to make recommendations without knowing what your repairing abilities amount to, but I can say, that putting a patch in the middle of a floor is not too bad (as opposed to a patch next-to or under a wall, where you will have to deal with C-channels, elevator bolts, etc.).
I would suggest that you remove whatever flooring that is between you and the subfloor, and get a good look at the soft spot. Jab at it with a sharp screwdriver or ice pick to get an idea of the size and shape of the rotten spot. As mentioned above, at this point, maybe penetrating epoxy will do the job for you, or if the wood is in too bad of shape, you could cut the rot out using an oscillating cutting tools (to avoid damaging frame or tanks that may be under the floor) and then use the piece you cut out as a pattern for a patch. Support the patch on the edges with "doublers" from underneath, if possible, or expand the area to land on frame members, etc.
If the above sounds outside your skill or interest, then you should take it to an RV shop. I imagine they repair rotten spots in floors routinely.
good luck!
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